Phagocytosis Affects Biguanide Sensitivity of Acanthamoeba spp
نویسندگان
چکیده
منابع مشابه
Phagocytosis affects biguanide sensitivity of Acanthamoeba spp.
The incidence of Acanthamoeba keratitis, a disease associated with contact lens wear, has been in apparent decline with the advent of multipurpose contact lens solutions. The concentrations of the biguanides chlorhexidine digluconate (CHX) and particularly polyhexamethylene biguanide (PHMB) included in multipurpose solutions (MPSs) are sublethal for amoebae. We evaluated by flow cytometry the e...
متن کاملDouble-Biguanide Therapy for Resistant Acanthamoeba Keratitis
AIMS To report the clinical and diagnostic findings of a patient with Acanthamoeba keratitis resistant to both polyhexamethylene biguanide (PHMB)-hexamidine and chlorhexidine-hexamidine treatment. METHODS Slit-lamp biomicroscopy, corneal cell scraping and histopathology were performed on a 39-year-old woman presenting with corneal ulcer in her left eye. RESULTS The patient was successfully ...
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Electron microscopic studies confirm and extend the conclusions derived previously from a quantitative biochemical study of the phagocytosis of polystyrene and polyvinyltoluene latex beads by Acanthamoeba (1). Latex beads 1.305, 1.90, and 2.68 micro in diameter are ingested individually, with each bead tightly surrounded by a membrane derived from the plasma membrane. Latex beads 0.557, 0.264, ...
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A method is described for the rapid and efficient isolation of phagocytic vesicles from large scale cultures of Acanthamoeba castellanii (Neff) that have been incubated with polystyrene latex beads. Cells were allowed to phagocytose latex beads for 30 min and then were homogenized, and the phagocytic vesicles were isolated by one centrifugation through several layers of sucrose. Identity and pu...
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ژورنال
عنوان ژورنال: Antimicrobial Agents and Chemotherapy
سال: 2002
ISSN: 0066-4804,1098-6596
DOI: 10.1128/aac.46.7.2069-2076.2002